GENE EXPRESSION PROFILING OF AGING AND CALORIC RESTRICTION ON THE
KIDNEY IN MICE USING OLIGONUCLEOTIDE ARRAY
Tsuyoshi Kayo1, Tomas A Prolla2 and Richard Weindruch13
1Wisconsin Regional Primate Research Center,
2Department of Genetics and Medical Genetics, and Department of Medicine, University of Wisconsin, Madison, WI 53705
3Geriatric Research, Education and Clinical Center, William S. Middleton VA Hospital, Madison, WI 53705
Aging of the kidney is characterized by a decline in renal function and an increase of age-related pathological changes in kidney structures, and is the major risk factor for several nephropathy complications of chronic age-related disease. It remains unknown the mechanisms of aging in kidney. Here we report the effects of aging and calorie restriction (CR) on the gene expression profile in the kidney from C57BL/6 mice through the use of oligonucleotide array. To determine the effect of age, each 5-month-old mouse (n=5) was compared to each 30-month-old mouse (n=5) by twenty-five pairwise comparisons for average fold change. Likewise, the effects of CR were determined by comparing each 30-month-old CR-fed mouse (n=5) to each 30-month-old control-fed mouse (n=5), generating twenty-five pairwise comparisons. Messenger RNA in kidney was conversed into cDNA. And Biotin-labeled cRNA were synthesized to hybridize to the Affymetrix Murine 11KsubA and 11KsubB Arrays of 13027 genes and expressed sequence tag (ESTs) which was derived from selected genes UniGene and GeneBank, and The institute for Genomic Research (TIGR) database. After washing,staining, and scanning,analysis was performed by an Affymetrix algorithm with Affymetrix GeneChip analysis software. Aging results in an up-regulation of transcripts involved in a variety of immunoglobulins and stress response, and a down-regulation of genes involved in metabolism. CR resulted in an up-regulation of transcripts involved in metabolism, and a down-regulation of genes involved in immunoglobulins and stress response. CR can partially or completely prevent the age-related transcription alterations, especially an up-regulation of transcripts involved in a variety of immunoglobulins appeared to show completely an effective CR prevention in kidney. These results suggest that gene expression profile provides that kidney aging is associated with specific transcriptional alterations and CR can alter age-related transcriptional changes, and also the induction of a variety of immunoglobulins may play important role in renal function for a kidney-specific with aging.
Key words:
Aging, Calorie restriction, Kidney, Oligonucleotide array
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