The Triad of Diet, Obesity, and Insulin Resistance: Molecular and Cellular Mechanisms





Andrew S. Greenberg, Susan K Fried

JM-USDA HNRCA at Tufts University, Boston, MA and University of Maryland, Baltimore, MD



One of the major metabolic problems facing aging individuals is the regulation of glucose homeostasis. Both insulin sensitivity and adequacy of insulin secretion can be affected during the aging process. Insulin sensitivity may be regulated and/or decreased by alterations in body composition. Increases in fat mass and/or fat redistribution may promote insulin resistance. Insulin resistance appears to be a central component of the metabolic syndrome, which appears to affect approximately 40% of individuals over the age of 60 years. The metabolic syndrome is increasing at an alarming rate and is a major risk factor for heart disease. As a result of alterations in body composition, adipocyte metabolism may become dysregulated. Enlarged fat cells produce less perilipin and adiponectin but increased quantities of interleukin-6 (IL-6) and tumor necrosis factor (TNF). Perilipin, which coats the surface of intracellular triacylglycerol in adipocytes, blocks the lipolytic actions of lipases. Enlarged adipocytes have reduced perilipin protein levels, which may facilitate increases in fatty acid release (FA). Increased circulating FA promotes insulin resistance in skeletal muscle and liver. Both adiponectin and IL-6 act as hormones released by adipocytes. Adiponectin increases oxidation of fatty acids in peripheral tissues. IL-6 increases adipocyte lipolysis and block insulin actions in peripheral tissues. Thus a reduction in adiponectin and increased production of IL-6 both promote insulin resistance. TNF appears to act locally in adipose tissue and increases adipocyte lipolysis. Regulation of fatty acid metabolism appears to be a critical regulator of insulin sensitivity. The mechanisms that underlie the reduction in insulin secretion with aging remain unclear. Thus actions to maintain fatty acid homeostasis are critical in preventing insulin resistance and countering any reduced insulin secretory capacity that may be associated with aging.




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